'Microbiology and Unknown Bacteria Lab\r'

'Unknown bacteria science lab Report Introduction The single-valued function to this lab was to identify an unknown bacteria from a mixed purification provided to us by our instructor. This study was d atomic number 53 by applying completely of the orders that have been instructed on and consequently far in microbiology laboratory class. sever all in ally test performed, provided us with some(a) come upon information about the unknown existence in question and how the bacteria function. all over a two week period, eighter prepared types of test media were provided to identify the assign unknown mixed cultures.\r\nNot all of these tests were performed on every culture, as some were employ only for gram affirmatory or gram forbid bacteria. The tests performed and what established a positive or negative test are as follows: Lab twenty-four hours 1; today in lab we obtained the unknown mixed culture â€Å"041”and one brain-heart infusion agar (BHIA). The starti ng time step was the preparation of the medium, the bottom of the BHIA deal was labeled with the bacterium number, initials, and section; then divided into four quarter-circles.\r\nThe second step, we used the septic technique to transfer a subtile amount of culture with a flame-sterilized inoculating eyelet to the first quadrant, flamed and cooled the loop again then transferred a small amount of the culture from the first quadrant to the second using the quadrant streaking method as illustrated on page 18 of the lab manual, repeating this process until all four quadrants were properly streaked.\r\nLab day 2; we collected our BHIA medium and began by identifying the morphology and cell-to cell arrangements of the colonies. Two different colonies were observed, the first habituation was yellow in color and larger in coat and the white colored colony was sparingly smaller in size. As instructed, all(prenominal) colony was prepared for gram staining, one slide for the large yel low colony and one for the smaller white colony. later properly gram staining the slides as directed in chapter six of the lab manual, the smears were examined under…\r\n'